Glutamate-pyruvate transaminase (GPT) polymorphism detected in human urine by isoelectric focusing.

Detection of recombinant human erythropoietin in urine by isoelectric focusing.

BACKGROUND Doping with erythropoietic proteins such as recombinant human erythropoietin (rHuEPO) and darbepoetin alfa is a serious issue in sport. There is little information on the time course of detection of rHuEPO in urine and on methods to evaluate electrophoresis-based data. METHODS We used a recently described isoelectric focusing method for detecting rHuEPO and endogenous EPO in urine ...

A cortisol-induced charge difference in desialylated human transcortin detected by isoelectric focusing.

Human desialylated transcortin was found to be microheterogeneous by isoelectric focusing. All samples tested showed two cortisol-binding components. Liganded with cortisol these components are isoelectric at pH 5.2 and 5.4. In the unliganded form, however, they eluted at pH 5.4 and 5.6, respectively. This cortisol-controlled transition was reversible in both directions. Isolated components con...

Isoelectric Focusing of Human Liver

1. Isoelectric focusing of human liver alkaline phosphatase in a sucrose density gradient with LKB Ampholine as carrier ampholytes is described. 2. Problems due to the chelating properties of the ampholytes and the pH gradient were examined. 3. A reactivation procedure to counter these effects was devised that can probably be used for other alkaline phosphatases. 4. The isoelectric point of hum...

Isoelectric focusing.

Capillary Isoelectric Focusing

Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press...